Activation Assay:Article Title: High-Dimensional Immune Profiling of Human Retinal Detachment Samples Using Spectral Flow Cytometry: A Protocol for Intraocular Immunotyping
Article Snippet: CD1c , L161 , Alexa Fluor 647 , BioLegend (331510) , Dendritic cells, NKT-Like cells , 1/20 , 5. .. NKG2A (CD159a) , REA110 , APC , Miltenyi (Bergisch Gladbach, Germany) (130-113-563) , NK, NKT-Like, and γδ T cell activation/differentiation , 1/50 , 2. .. CD38 , HIT2 , APC/Fire810 , BioLegend (356644) , Monocyte, dendritic cell, T cell, and B cell activation/differentiation , 1/40 , 2.5.
Cell Stimulation:Article Title: Antigen Format Determines Immunogenicity of AAV-Based SARS-CoV-2 Vaccines: Full-Length Spike Versus Truncated Subunits
Article Snippet: .. Reagents for T-Cell Stimulation Assays PepTivator® SARS-CoV-2 Prot_S peptide pool was obtained from Miltenyi Biotec, Bergisch Gladbach, Germany (Cat. No. 130-126-701). .. Anti-mouse CD28 costimulatory antibody was purchased from BioLegend, San Diego, CA, USA (Cat. No. 102105).
Shear:Article Title: Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction.
Article Snippet: .. For Review Only Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction Supplementary materials and data analyses Reagents and materials used: Reagent/materials Vendor Catalog number Notes Mouse lung dissociation kit Miltenyi Biotec., Bergisch Gladbach, Germany #130-095-927 RIPA Buffer Invitrogen., Carlsbad, CA #1861278 HALTTM phosphatase inhibitor cocktail Invitrogen., Carlsbad, CA #1862495 HUVECs Lonza., Morristown, NJ #CC-2519 ON-TARGETplus SiRNA CD44 (Human) Dharmacon Reagents., Cambridge, United Kingdom #L-009999-000010 LipofectamineTM RNAiMAX transfection reagent Invitrogen., Carlsbad, CA #13778150 Opti-MEMTM medium Gibco., Grand Island, NY #11058021 phorbol 12-myristate 13-acetate Sigma-aldrich., St. Louis, MO #524400 Ad. hADAM17 Vector biolabs., Devault, PA ADV-200349 #2001 Ad-CMV-null Vector biolabs., Devault, PA #1300 Recombinant active ADAM17 R&D Systems., Minneapolis, MN #930-ADB-010 Ibidi μ-Slide VI 0.4 Luer channels Ibidi., Fitchburg, WI #80186 Ibidi μ-Slide I 0.6 Luer channels Ibidi., Fitchburg, WI #80606 Fluo-4 Assay Kit Calcium Abcam., Cambridge, United Kingdom #ab228555 Hyaluronidase from bovine testes Sigma-aldrich., St. Louis, MO #H3506 High-capacity carboxyl sensors Nicoya., Kitchener, Canada #SEN-AU100-10-HC- COOH Page 34 of 38 https://mc.manuscriptcentral.com/function Manuscripts submitted to Function 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Downloaded from journals.physiology.org/journal/function (2001:448A:5160:33DE:ADDE:2129:780F:130F) on March 31, 2026. .. For Review Only Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction Protein A Nicoya., Kitchener, Canada #SEN-AU100-10-PROA- KIT Recombinant human CD44-fc chimera R&D Systems., Minneapolis, MN #3660-CD Hyaluronan Lifecore biomedical., Chaska, MN #HA100K-1 Component C Anaspec., Fremont, CA #AS-72085 SensoLyte TACE activity kit Anaspec., Fremont, CA #AS-72085 Human hyaluronan ELISA kit R&D Systems., Minneapolis, MN #DHYAL0 15-well 3D Ibidi μSlides Ibidi., Fitchburg, WI #81506 Antibodies and stains used: Antibodies/stains Working concentration Vendor Catalog RRID Fluo-4 Assay Kit Calcium 1X Abcam., Cambridge, United Kingdom #ab228555 - Anti-mouse CD44LE/AF KM201 antibody – Functional experiment (i.e., FMD) 0.25mg/mL Southern Biotech., Birmingham Alabama #1500-14 RRID:AB_2794779 IgG1-LE/AF isotype control – Functional experiment (i.e., FMD) 0.25mg/mL Southern Biotech., Birmingham Alabama #0116-14 RRID:AB_2793987 CD44 (MEM-263) 1:100 Abcam., Cambridge, United Kingdom #9524 RRID:AB_307310 Anti-ADAM17 1:100 LSbio., Saratoga, CA #B4426 RRID:AB_10796856 Anti-ADAM17 1:100 Abcam., Cambridge, United Kingdom #39162 RRID:AB_722565 Page 35 of 38 https://mc.manuscriptcentral.com/function Manuscripts submitted to Function 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Downloaded from journals.physiology.org/journal/function (2001:448A:5160:33DE:ADDE:2129:780F:130F) on March 31, 2026.
Transfection:Article Title: Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction.
Article Snippet: .. For Review Only Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction Supplementary materials and data analyses Reagents and materials used: Reagent/materials Vendor Catalog number Notes Mouse lung dissociation kit Miltenyi Biotec., Bergisch Gladbach, Germany #130-095-927 RIPA Buffer Invitrogen., Carlsbad, CA #1861278 HALTTM phosphatase inhibitor cocktail Invitrogen., Carlsbad, CA #1862495 HUVECs Lonza., Morristown, NJ #CC-2519 ON-TARGETplus SiRNA CD44 (Human) Dharmacon Reagents., Cambridge, United Kingdom #L-009999-000010 LipofectamineTM RNAiMAX transfection reagent Invitrogen., Carlsbad, CA #13778150 Opti-MEMTM medium Gibco., Grand Island, NY #11058021 phorbol 12-myristate 13-acetate Sigma-aldrich., St. Louis, MO #524400 Ad. hADAM17 Vector biolabs., Devault, PA ADV-200349 #2001 Ad-CMV-null Vector biolabs., Devault, PA #1300 Recombinant active ADAM17 R&D Systems., Minneapolis, MN #930-ADB-010 Ibidi μ-Slide VI 0.4 Luer channels Ibidi., Fitchburg, WI #80186 Ibidi μ-Slide I 0.6 Luer channels Ibidi., Fitchburg, WI #80606 Fluo-4 Assay Kit Calcium Abcam., Cambridge, United Kingdom #ab228555 Hyaluronidase from bovine testes Sigma-aldrich., St. Louis, MO #H3506 High-capacity carboxyl sensors Nicoya., Kitchener, Canada #SEN-AU100-10-HC- COOH Page 34 of 38 https://mc.manuscriptcentral.com/function Manuscripts submitted to Function 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Downloaded from journals.physiology.org/journal/function (2001:448A:5160:33DE:ADDE:2129:780F:130F) on March 31, 2026. .. For Review Only Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction Protein A Nicoya., Kitchener, Canada #SEN-AU100-10-PROA- KIT Recombinant human CD44-fc chimera R&D Systems., Minneapolis, MN #3660-CD Hyaluronan Lifecore biomedical., Chaska, MN #HA100K-1 Component C Anaspec., Fremont, CA #AS-72085 SensoLyte TACE activity kit Anaspec., Fremont, CA #AS-72085 Human hyaluronan ELISA kit R&D Systems., Minneapolis, MN #DHYAL0 15-well 3D Ibidi μSlides Ibidi., Fitchburg, WI #81506 Antibodies and stains used: Antibodies/stains Working concentration Vendor Catalog RRID Fluo-4 Assay Kit Calcium 1X Abcam., Cambridge, United Kingdom #ab228555 - Anti-mouse CD44LE/AF KM201 antibody – Functional experiment (i.e., FMD) 0.25mg/mL Southern Biotech., Birmingham Alabama #1500-14 RRID:AB_2794779 IgG1-LE/AF isotype control – Functional experiment (i.e., FMD) 0.25mg/mL Southern Biotech., Birmingham Alabama #0116-14 RRID:AB_2793987 CD44 (MEM-263) 1:100 Abcam., Cambridge, United Kingdom #9524 RRID:AB_307310 Anti-ADAM17 1:100 LSbio., Saratoga, CA #B4426 RRID:AB_10796856 Anti-ADAM17 1:100 Abcam., Cambridge, United Kingdom #39162 RRID:AB_722565 Page 35 of 38 https://mc.manuscriptcentral.com/function Manuscripts submitted to Function 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Downloaded from journals.physiology.org/journal/function (2001:448A:5160:33DE:ADDE:2129:780F:130F) on March 31, 2026.
Recombinant:Article Title: Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction.
Article Snippet: .. For Review Only Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction Supplementary materials and data analyses Reagents and materials used: Reagent/materials Vendor Catalog number Notes Mouse lung dissociation kit Miltenyi Biotec., Bergisch Gladbach, Germany #130-095-927 RIPA Buffer Invitrogen., Carlsbad, CA #1861278 HALTTM phosphatase inhibitor cocktail Invitrogen., Carlsbad, CA #1862495 HUVECs Lonza., Morristown, NJ #CC-2519 ON-TARGETplus SiRNA CD44 (Human) Dharmacon Reagents., Cambridge, United Kingdom #L-009999-000010 LipofectamineTM RNAiMAX transfection reagent Invitrogen., Carlsbad, CA #13778150 Opti-MEMTM medium Gibco., Grand Island, NY #11058021 phorbol 12-myristate 13-acetate Sigma-aldrich., St. Louis, MO #524400 Ad. hADAM17 Vector biolabs., Devault, PA ADV-200349 #2001 Ad-CMV-null Vector biolabs., Devault, PA #1300 Recombinant active ADAM17 R&D Systems., Minneapolis, MN #930-ADB-010 Ibidi μ-Slide VI 0.4 Luer channels Ibidi., Fitchburg, WI #80186 Ibidi μ-Slide I 0.6 Luer channels Ibidi., Fitchburg, WI #80606 Fluo-4 Assay Kit Calcium Abcam., Cambridge, United Kingdom #ab228555 Hyaluronidase from bovine testes Sigma-aldrich., St. Louis, MO #H3506 High-capacity carboxyl sensors Nicoya., Kitchener, Canada #SEN-AU100-10-HC- COOH Page 34 of 38 https://mc.manuscriptcentral.com/function Manuscripts submitted to Function 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Downloaded from journals.physiology.org/journal/function (2001:448A:5160:33DE:ADDE:2129:780F:130F) on March 31, 2026. .. For Review Only Endothelial CD44 cleavage by ADAM17 impairs shear stress mechanotransduction Protein A Nicoya., Kitchener, Canada #SEN-AU100-10-PROA- KIT Recombinant human CD44-fc chimera R&D Systems., Minneapolis, MN #3660-CD Hyaluronan Lifecore biomedical., Chaska, MN #HA100K-1 Component C Anaspec., Fremont, CA #AS-72085 SensoLyte TACE activity kit Anaspec., Fremont, CA #AS-72085 Human hyaluronan ELISA kit R&D Systems., Minneapolis, MN #DHYAL0 15-well 3D Ibidi μSlides Ibidi., Fitchburg, WI #81506 Antibodies and stains used: Antibodies/stains Working concentration Vendor Catalog RRID Fluo-4 Assay Kit Calcium 1X Abcam., Cambridge, United Kingdom #ab228555 - Anti-mouse CD44LE/AF KM201 antibody – Functional experiment (i.e., FMD) 0.25mg/mL Southern Biotech., Birmingham Alabama #1500-14 RRID:AB_2794779 IgG1-LE/AF isotype control – Functional experiment (i.e., FMD) 0.25mg/mL Southern Biotech., Birmingham Alabama #0116-14 RRID:AB_2793987 CD44 (MEM-263) 1:100 Abcam., Cambridge, United Kingdom #9524 RRID:AB_307310 Anti-ADAM17 1:100 LSbio., Saratoga, CA #B4426 RRID:AB_10796856 Anti-ADAM17 1:100 Abcam., Cambridge, United Kingdom #39162 RRID:AB_722565 Page 35 of 38 https://mc.manuscriptcentral.com/function Manuscripts submitted to Function 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Downloaded from journals.physiology.org/journal/function (2001:448A:5160:33DE:ADDE:2129:780F:130F) on March 31, 2026.
Article Title: Epididymitis promotes formation of tertiary lymphoid organs in the cauda epididymidis.
Article Snippet: .. Clones Final Dilution Recombinant monoclonal anti-mouse CD19 PEVio770 REAfinity conjugated Miltenyi Biotec, Bergisch Gladbach, Germany 130-112- 037 REA749 1:50 .. Rat monoclonal anti-AID Invitrogen, Oregon, USA 14-5959- 82 1:30 Rat monoclonal anti-CD138 BioLegend, San Diego, CA, USA 142501 1:100 Rabbit monoclonal anti-CD19 Abcam, Cambridge, UK ab245235 1:50 Rat monclonal anti-CD3ε Cell Signaling Technology, Danvers, Massachusetts, USA 4443 1:50 Rabbit monoclonal anti-CD45 Cell Signaling Technology D3F8Q 1:50 Goat polyclonal anti- CXCL13 R&D Systems, Minneapolis, Minnesota, USA PA547018 1:20 Rat monoclonal anti- F4/80 Cell Signaling Technology 71299 1:100 Rat monoclonal anti-MECA79 BioLegend, San Diego, CA, USA 120801 1:100 Rat monoclonal anti-MECA79 Alexa Fluor 647 BioLegend 120807 1:100 Mouse monoclonal antiPCNA BioLegend 307901 1:50 Goat polyclonal antipodoplanin R&D Systems, Minneapolis AF3244 1:20 Mouse monoclonal anti-CD45 Dianova GmbH, Hamburg, Germany DLN083890 1:30 Mouse monoclonal antiCD20cy Agilent Technologies, Santa Clara, CA, USA M0755012 1:50 Rabbit polyclonal anti-CD3 Agilent A0452292 1:50 Pure Arachis hypogaea lectin (PNA), FITC conjugated EY Laboratories, San Mateo, USA F-2301-1 1:200 Secondary Antibodies Goat anti-rat IgG (H+L) Alexa Fluor 546 conjugated Invitrogen, Oregon, USA A-11081 1:2000 Goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugated Invitrogen A-11034 1:2000 Goat anti-rabbit IgG (H+L) Alexa Fluor 546 conjugated Invitrogen A-11008 1:2000 Goat anti-rat IgG (H+L) Highly Cross-Adsorbed Alexa Fluor 488 conjugated Invitrogen A48262 1:1000 Donkey anti-goat (H+L) Alexa Fluor 488 conjugated abcam ab150129 1:1000 Donkey anti-mouse IgG (H+L) Highly Cross-Adsorbed Alexa Fluor 488 conjugated Invitrogen A21202 1:1000 Donkey anti-rat IgG (H+L) Highly Cross-Adsorbed Alexa Fluor Plus 647 Conjugated Invitrogen A48272 1:500 Goat anti-mouse IgG F(ab)2 Alexa Fluor 488 conjugated Jackson ImmunoResearch Labs, West Grove, USA 115-546- 072 1:800
Magnetic Cell Separation:Article Title: Correlation of molecular and cellular signatures in primary skeletal muscle satellite cells derived from lean and diet-induced obese mice
Article Snippet: Integrin α7 (Itga7) , Abcam, Cambridge, UK; ab203254 , Rabbit, polyclonal , WB, 1:1000. .. Integrin α7-PE-Vio770 (Itga7) , Miltenyi Biotec, Bergisch Gladbach, Germany; 130-120−812 , Mouse, monoclonal , MACS, FACS. .. Pax7 , Thermo Scientific, Munich, Germany; PA1-117 , Rabbit, polyclonal , WB, 1:1000.
FACS:Article Title: Correlation of molecular and cellular signatures in primary skeletal muscle satellite cells derived from lean and diet-induced obese mice
Article Snippet: Integrin α7 (Itga7) , Abcam, Cambridge, UK; ab203254 , Rabbit, polyclonal , WB, 1:1000. .. Integrin α7-PE-Vio770 (Itga7) , Miltenyi Biotec, Bergisch Gladbach, Germany; 130-120−812 , Mouse, monoclonal , MACS, FACS. .. Pax7 , Thermo Scientific, Munich, Germany; PA1-117 , Rabbit, polyclonal , WB, 1:1000.
Clone Assay:Article Title: Epididymitis promotes formation of tertiary lymphoid organs in the cauda epididymidis.
Article Snippet: .. Clones Final Dilution Recombinant monoclonal anti-mouse CD19 PEVio770 REAfinity conjugated Miltenyi Biotec, Bergisch Gladbach, Germany 130-112- 037 REA749 1:50 .. Rat monoclonal anti-AID Invitrogen, Oregon, USA 14-5959- 82 1:30 Rat monoclonal anti-CD138 BioLegend, San Diego, CA, USA 142501 1:100 Rabbit monoclonal anti-CD19 Abcam, Cambridge, UK ab245235 1:50 Rat monclonal anti-CD3ε Cell Signaling Technology, Danvers, Massachusetts, USA 4443 1:50 Rabbit monoclonal anti-CD45 Cell Signaling Technology D3F8Q 1:50 Goat polyclonal anti- CXCL13 R&D Systems, Minneapolis, Minnesota, USA PA547018 1:20 Rat monoclonal anti- F4/80 Cell Signaling Technology 71299 1:100 Rat monoclonal anti-MECA79 BioLegend, San Diego, CA, USA 120801 1:100 Rat monoclonal anti-MECA79 Alexa Fluor 647 BioLegend 120807 1:100 Mouse monoclonal antiPCNA BioLegend 307901 1:50 Goat polyclonal antipodoplanin R&D Systems, Minneapolis AF3244 1:20 Mouse monoclonal anti-CD45 Dianova GmbH, Hamburg, Germany DLN083890 1:30 Mouse monoclonal antiCD20cy Agilent Technologies, Santa Clara, CA, USA M0755012 1:50 Rabbit polyclonal anti-CD3 Agilent A0452292 1:50 Pure Arachis hypogaea lectin (PNA), FITC conjugated EY Laboratories, San Mateo, USA F-2301-1 1:200 Secondary Antibodies Goat anti-rat IgG (H+L) Alexa Fluor 546 conjugated Invitrogen, Oregon, USA A-11081 1:2000 Goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugated Invitrogen A-11034 1:2000 Goat anti-rabbit IgG (H+L) Alexa Fluor 546 conjugated Invitrogen A-11008 1:2000 Goat anti-rat IgG (H+L) Highly Cross-Adsorbed Alexa Fluor 488 conjugated Invitrogen A48262 1:1000 Donkey anti-goat (H+L) Alexa Fluor 488 conjugated abcam ab150129 1:1000 Donkey anti-mouse IgG (H+L) Highly Cross-Adsorbed Alexa Fluor 488 conjugated Invitrogen A21202 1:1000 Donkey anti-rat IgG (H+L) Highly Cross-Adsorbed Alexa Fluor Plus 647 Conjugated Invitrogen A48272 1:500 Goat anti-mouse IgG F(ab)2 Alexa Fluor 488 conjugated Jackson ImmunoResearch Labs, West Grove, USA 115-546- 072 1:800
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